room temperature (Proteintech)
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Proteintech
room temperature
Room Temperature, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+collagen+type+ii/Collagen+Type+II+Antibody/pmc12963903-85-79-93
Average 96 stars, based on 584 article reviews
Room Temperature, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+collagen+type+ii/Collagen+Type+II+Antibody/pmc12963903-85-79-93
Average 96 stars, based on 584 article reviews
room temperature - by Bioz Stars,
2026-09
96/100 stars
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Staining:Article Title: microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3. Article Snippet: .. The primary antibodies for immunocytofluorescence staining included the following: Article Title: microRNA‐365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3 Article Snippet: .. The primary antibodies for immunocytofluorescence staining included the following: Article Title: microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3. Article Snippet: .. The primary antibodies for immunohistofluorescence staining included the following: Article Title: microRNA‐365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3 Article Snippet: .. The primary antibodies for immunohistofluorescence staining included the following: Immunohistofluorescence:Article Title: microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3. Article Snippet: .. The primary antibodies for immunohistofluorescence staining included the following: Article Title: microRNA‐365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3 Article Snippet: .. The primary antibodies for immunohistofluorescence staining included the following: Incubation:Article Title: An anti-senescence hydrogel with pH-responsive drug release for mitigating intervertebral disc degeneration and low back pain Article Snippet: After removing the working solution, three random fields of each group were imaged under an optical microscope and the proportion of β-Gal + cells were calculated using image J software (version: 1.52n, author: Wayne Rasband). .. At the designated time point, the cells were washed with PBS solution, fixed with 4 % paraformaldehyde for 15 min, followed by infiltration with 0.5 % Triton X-100 for 10 min. After being blocked with 5 % bovine serum albumin for 1 h, the samples were incubated with a primary antibody overnight at 4 °C (anti-aggrecan: 13880-1-AP, Proteintech, Wuhan, China, 1:400, 1.25 μg/mL; Article Title: The role and underlying mechanism of dental pulp stem cell-derived exosomal miR-31 in the treatment of osteoarthritis by targeting mTOR to enhance chondrocyte autophagy levels Article Snippet: Immunohistochemistry staining was done using the SP-9000 Histostain-plus kit (Thermo Fisher) as per the manufacturer’s recommendation. .. Tissue sections were incubated at 4°C overnight with the following antibodies: Saline:Article Title: Injectable chitosan microspheres resisting inflammatory and oxidative stress for ameliorating intervertebral disc degeneration Article Snippet: The treatment of intervertebral disc (IVD) degeneration remains a significant challenge due to the unique ischemic structure of the IVD, which comprises the scavenging of inflammatory cytokines, alleviation of cellular oxidative stress responses, restoration of nuclei pulposus (NP) cell viability, and recovery of IVD biomechanical function.. Herein, we developed an injectable microsphere (CS-MnO2@PC) by incorporating chitosan microspheres (CS) with manganese dioxide (MnO2) nanozymes and celecoxib encapsulated in Pluronic F-127 (PC) nanosized micelles, via in situ redox or Schiff base reaction.. The hybrid carrier demonstrates robust capabilities in scavenging free radicals, alleviating extracellular oxidative stress, and reducing inflammatory cytokines in NP cells, as evidenced by RT-qPCR and immunofluorescence staining assays. |
![RA-Specific Serum IgG Antibodies in DBA/1J Mice. Sera were collected biweekly from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 9–18; non-arthritic: N = 17–37), or PBS (N = 14) and tested via indirect <t>ELISA</t> for A) anti-CitP, and B) anti-HomoCitP IgG antibodies. Graphs show the median [IQR] for each group, with a cut-off indicating the limit of detection (dashed line). Statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. The resulting p-values are indicated on the graphs.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0342/pmc12810342/pmc12810342__gr6.jpg)
